HLA-DNA-DQ typing by PCR-RFLP method in renal transplantation
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چکیده
منابع مشابه
Improvement of sensitivity in HLA-DRB1 typing by semi-nested PCR-RFLP.
A sensitive method of HLA-DRB1 typing was devised using a semi-nested polymerase chain reaction (PCR) followed by a restriction fragment length polymorphism (RFLP) analysis (semi-nested PCR-RFLP method). The first-round amplification (30 cycles) of the semi-nested PCR was performed using DRB generic primer pairs and the second round of PCRs (20 cycles) were performed using DRB1 group-specific p...
متن کاملTyping class I HLA-A gene using a nested PCR-RFLP procedure.
In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial ...
متن کاملComparison of cattle BoLA-DRB3 typing by PCR-RFLP, direct sequencing, and high-resolution DNA melting curve analysis
Major histocompatibility complex (MHC) represents an important genetic marker for manipulation to improve the health and productivity of cattle. It is closely associated with numerous disease susceptibilities and immune responses. Bovine MHC, also called bovine leukocyte antigen (BoLA), is considered as a suitable marker for genetic diversity studies. In cattle, most of the polymorphisms are lo...
متن کاملClinical features of sarcoidosis in relation to HLA distribution and HLA-DRB3 genotyping by PCR-RFLP.
BACKGROUND Susceptibility to the development of sarcoidosis has been demonstrated to be associated with HLA-DR5, -DR6, and -DR8 encoded by the DRB1 gene. However, involvement of the DRB3 (HLA-DR52) gene in the development of sarcoidosis remains unclear. METHODS HLA-DRB3 genotyping was performed using the PCR-RFLP method and the clinical features of the patients with and without the DR3, 5, 6,...
متن کاملHLA-G genetic polymorphism in 57 Portuguese white families studied by PCR-RFLP and PCR-SSOP.
FROM STUDIES on tissue-specific expression and interaction with T and NK cells, it was suggested that HLA-G may have an important role in immunologic tolerance. The recognition of HLA-G polymorphism, its association with other classical HLA class I loci, and eventual variation among different populations may prove to be useful in understanding its immunologic functions and evolution of human po...
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ژورنال
عنوان ژورنال: Okayama Igakkai Zasshi (Journal of Okayama Medical Association)
سال: 1993
ISSN: 0030-1558,1882-4528
DOI: 10.4044/joma1947.105.7-8_715